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biomarkers limonene  (KCAS Bioanalytical and Biomarker Services)
94
KCAS Bioanalytical and Biomarker Services biomarkers limonene
Biomarkers Limonene, supplied by KCAS Bioanalytical and Biomarker Services, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/standard+procedures/10__1016_slash_j__rechem__2026__103226-241-5-4?v=KCAS+Bioanalytical+and+Biomarker+Services
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glycerol  (Chem Impex International)
95
Chem Impex International glycerol
Glycerol, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β actin internal standard for normalization  (Revvity)
90
Revvity β actin internal standard for normalization
Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the <t>β-actin</t> PCR product of 289 bp as an internal standard.
β Actin Internal Standard For Normalization, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/standard+procedures/pmc06782395-133-67-6?v=Revvity
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β actin internal standard for normalization - by Bioz Stars, 2026-07
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hybrid immunocapture lc ms ms  (KCAS Bioanalytical and Biomarker Services)
93
KCAS Bioanalytical and Biomarker Services hybrid immunocapture lc ms ms
Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the <t>β-actin</t> PCR product of 289 bp as an internal standard.
Hybrid Immunocapture Lc Ms Ms, supplied by KCAS Bioanalytical and Biomarker Services, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hybrid immunocapture lc ms ms - by Bioz Stars, 2026-07
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kcas bio analytical  (KCAS Bioanalytical and Biomarker Services)
99
KCAS Bioanalytical and Biomarker Services kcas bio analytical
Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the <t>β-actin</t> PCR product of 289 bp as an internal standard.
Kcas Bio Analytical, supplied by KCAS Bioanalytical and Biomarker Services, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/standard+procedures/pm40684393-96-17-17?v=KCAS+Bioanalytical+and+Biomarker+Services
Average 99 stars, based on 1 article reviews
kcas bio analytical - by Bioz Stars, 2026-07
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us epa priority pah standards  (ZeptoMetrix corporation)
90
ZeptoMetrix corporation us epa priority pah standards
Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the <t>β-actin</t> PCR product of 289 bp as an internal standard.
Us Epa Priority Pah Standards, supplied by ZeptoMetrix corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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agar plate  (Teknova)
90
Teknova agar plate
Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the <t>β-actin</t> PCR product of 289 bp as an internal standard.
Agar Plate, supplied by Teknova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/standard+procedures/pm33784496-614-47-55?v=Teknova
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agar plate - by Bioz Stars, 2026-07
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internal standard operating procedures and quality assurance/ quality control protocols  (geological survey network)
90
geological survey network internal standard operating procedures and quality assurance/ quality control protocols
Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the <t>β-actin</t> PCR product of 289 bp as an internal standard.
Internal Standard Operating Procedures And Quality Assurance/ Quality Control Protocols, supplied by geological survey network, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/standard+procedures/pm27699830-118-23-16?v=geological+survey+network
Average 90 stars, based on 1 article reviews
internal standard operating procedures and quality assurance/ quality control protocols - by Bioz Stars, 2026-07
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internal standard operating procedure (sop)  (AbbVie Inc)
90
AbbVie Inc internal standard operating procedure (sop)
Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the <t>β-actin</t> PCR product of 289 bp as an internal standard.
Internal Standard Operating Procedure (Sop), supplied by AbbVie Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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internal standard operating procedure (sop) - by Bioz Stars, 2026-07
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standard procedures for series-1 soil analysis  (Agvise Laboratories)
90
Agvise Laboratories standard procedures for series-1 soil analysis
Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the <t>β-actin</t> PCR product of 289 bp as an internal standard.
Standard Procedures For Series 1 Soil Analysis, supplied by Agvise Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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standard procedures for series-1 soil analysis - by Bioz Stars, 2026-07
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broth microdilution procedure for susceptibility testing of brucella spp  (Clinical and Laboratory Standards Institute)
90
Clinical and Laboratory Standards Institute broth microdilution procedure for susceptibility testing of brucella spp
Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the <t>β-actin</t> PCR product of 289 bp as an internal standard.
Broth Microdilution Procedure For Susceptibility Testing Of Brucella Spp, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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broth microdilution procedure for susceptibility testing of brucella spp - by Bioz Stars, 2026-07
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broth microdilution procedure  (Clinical and Laboratory Standards Institute)
90
Clinical and Laboratory Standards Institute broth microdilution procedure
Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the <t>β-actin</t> PCR product of 289 bp as an internal standard.
Broth Microdilution Procedure, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/standard+procedures/us11236314-1430-28-18?v=Clinical+and+Laboratory+Standards+Institute
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broth microdilution procedure - by Bioz Stars, 2026-07
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Image Search Results


Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the β-actin PCR product of 289 bp as an internal standard.

Journal: The Journal of Neuroscience

Article Title: Neuroprotection and Neuronal Differentiation Studies Using Substantia Nigra Dopaminergic Cells Derived from Transgenic Mouse Embryos

doi: 10.1523/JNEUROSCI.19-01-00010.1999

Figure Lengend Snippet: Detection of TH expression and DA neuronal markers by Western blot analysis and RT-PCR. A, The representative DA marker, TH expression, was confirmed by Western blot analysis after maintaining the SN4741 cells for a week in high-density culture at 37°C (lane 4) and 33°C (lane 5). The 62 kDa TH band in the SN4741 cells was consistent with the molecular weight of TH isolated from mouse adrenal gland (lane 1) and similarly derived locus coeruleus noradrenergic cell line, grown at 37°C (lane 2) and 33°C (lane 3), which were used as positive controls for the Western blot analysis. Each lane contained 15 μg of protein except lane 1 (5 μg) and lane 2 (30 μg). The specific amount of TH protein in the SN4741 cells grown at 37°C was higher than the culture at 33°C. As demonstrated in a TH-positive pheochromocytoma cell, PC12, culture (Kim et al., 1995), our SN DA cell line also exhibited the increase in TH expression in the high cell density during a prolonged culture (7–10 d). B, The expression of some DA neuron-specific markers, such as NT-3, BDNF, DA-T, and D2R were detected by RT-PCR in the SN4741 cell line. After Southern blotting of both 1 and 5 μl of each RT-PCR sample, the presence of each specific size band (the strongest band) was detected and confirmed with radiolabeled human BDNF cDNA, rat NT-3 cDNA, mouse DA-T cDNA, or mouse D2R oligonucleotide probe, respectively. Each filter was hybridized with each specific probe and exposed separately. The expected major band sizes were 430 bp for NT-3, 407 bp for BDNF, 553 bp for DA-T, and 490 bp for D2R. β represents the β-actin PCR product of 289 bp as an internal standard.

Article Snippet: PCR was performed with Taq polymerase (Perkin-Elmer, Norwalk, CT) using an appropriate primer sets for BDNF (nucleotides 374–393 and 761–780; GenBank accession number G287898), neurotrophin-3 (NT-3, nucleotides 441–460 and 851–870; GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"X53257","term_id":"53451"}} X53257 ), D 2 autoreceptor (D 2 R, nucleotides 1581–1600 and 2051–2070; GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"X55674","term_id":"50648"}} X55674 ), and dopamine transporter (DA-T, nucleotides 2381–2400 and 6241–6250; GenBank Accession number {"type":"entrez-nucleotide","attrs":{"text":"U15791","term_id":"338819863"}} U15791 ), a β-actin internal standard for normalization (Gene Link, Thornwood, NY), trace amounts of [ 32 P]dCTP, and various amounts of cDNA.

Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Marker, Molecular Weight, Isolation, Derivative Assay, Southern Blot